Evaluating Gibberellic Acid-Induced Seed Dormancy Release in Rice (Oryza sativa L.) for Enhanced Germination
DOI:
https://doi.org/10.69569/jip.2025.383Keywords:
Gibberellic acid (GA3), Phytohormones, Rice, Seed dormancy, Seed germination, Seedling emergenceAbstract
Seed dormancy is a significant challenge in rice production, leading to uneven and poor germination rates. This study examines the effectiveness of gibberellic acid (GA3) in enhancing rice seed germination by alleviating seed dormancy. The experiment was conducted from April to May 2024 at Barangay Mabini, Santo Domingo, Nueva Ecija, using a Completely Randomized Design (CRD) with six treatments (Control, 50 ppm, 75 ppm, 100 ppm, 150 ppm, and 200 ppm GA3) and three replications. The primary objectives were to assess the impact of varying GA3 concentrations on seed dormancy release in an inbred rice variety (NSIC Rc 160), determine the optimal concentration for maximum germination rates, and compare the germination and seedling emergence performance of GA3-treated seeds with that of untreated seeds. The study analyzed critical germination data such as percent germination, and percent of seedling emergence, time to 10% germination and emergence (T10), mean germination and emergence time (T50), time to 90% germination and emergence (T90), and mean spread of germination and emergence time (T90-T10). The results showed that GA3 treatments at up to 150 ppm significantly increased germination rates compared to the control. The 50 ppm GA3 treatment was remarkably efficient, resulting in a 100% germination rate in one setup and the fastest T10. Higher concentrations (200 ppm) were shown to inhibit germination while significantly increasing T10, T50, and T90. Optimal germination enhancement was observed at concentrations ranging from 50 to 150 ppm, with 50 ppm showing the most consistent improvement across all parameters. The study also found that GA3 concentrations significantly improved the uniformity of germination and emergence times in rice cultivation. The 75 ppm treatment improved germination uniformity slightly, whereas higher concentrations (100 and 200 ppm) resulted in a slight increase in spread. The ideal concentration for increasing emergence uniformity was around 150 ppm. This study demonstrates that optimal GA3 concentrations can effectively break seed dormancy and enhance rice germination rates, providing valuable insights for improving rice production and food security. These results show that GA₃ can improve germination performance and seedling uniformity by breaking dormancy. The use of ideal GA₃ concentrations has the potential to enhance seed technology and promote sustainable rice production by facilitating more effective rice establishment.
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References
Adhikari, S., & Subedi, R. (2022). Effect of seed priming agents (GA3, PEG, hydropriming) in the early development of maize. RJOAS, 9(129), 113. https://doi.org/10.18551/rjoas.2022-09.11
Amorim, D. J., dos Santos, A. R. P., da Piedade, G. N., de Faria, R. Q., da Silva, E. A. A., & Sartori, M. M. P. (2021). The use of the generalized linear model to assess the speed and uniformity of germination of corn and soybean seeds.
Chen, D., Zou, W., Zhang, M., Liu, J., Chen, L., Peng, T., & Ye, G. (2023). Genome-wide association study for seed dormancy using re-sequenced germplasm under multiple conditions in rice. International Journal of Molecular Sciences, 24(7), 6117. https://doi.org/10.3390/ijms24076117
Chiboub, B., Maatougui, A., Aboukhali, K., Otouya, S., Zarqi, F., Nazih, A., & Baghour, M. (2024). Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L. Journal of Applied and Natural Science, 16(1). https://doi.org/10.31018/jans.v16i1.5287
Ebone, L. A., Caverzan, A., Tagliari, A., Chiomento, J. L. T., Silveira, D. C., & Chavarria, G. (2020). Soybean seed vigor: Uniformity and growth as key factors to improve yield. Agronomy, 10(4), 545. https://doi.org/10.3390/agronomy10040545
Erol, S. A., & Arslanoğlu, Ş. F. (2022). The effect of different gibberellic acid (GA3) doses on seed germination properties of some soybean [Glycine max (L.) merr.] cultivars. Uluslararası Tarım Araştırmalarında Yenilikçi Yaklaşımlar Dergisi (International Journal of Innovative Approaches in Agricultural Research), 6(4), 340-350. https://doi.org/10.29329/ijiaar.2022.506.5
Gong, D., He, F., Liu, J., Zhang, C., Wang, Y., Tian, S., Sun, C., & Zhang, X. (2022). Understanding of hormonal regulation in rice seed germination. Life (Basel), 12(7), 1021. https://doi.org/10.3390/life12071021
Huang, Y., Song, J., Hao, Q., Mou, C., Wu, H., Zhang, F., Zhu, Z., Wang, P., Ma, T., Fu, K., Chen, Y., Nguyen, T., Liu, S., Jiang, L., & Wan, J. (2023). Weak seed dormancy 1, an aminotransferase protein, regulates seed dormancy in rice through the GA and ABA pathways. Plant Physiology and Biochemistry, 202, 107923. https://doi.org/10.1016/j.plaphy.2023.107923
Li, Q., & Yang, A. (2020). Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature. Environmental and Experimental Botany, 178, 104216.
https://doi.org/10.1016/j.envexpbot
Mohammed, A. (2023). Effect of gibberellic acid on germination and seedling growth of soybean (Glycine max L. Merrill). Revista Boliviana, 8(2), 41. https://doi.org/10.21931/RB/2023.08.02.41
Ritonga, F. N., Zhou, D., Zhang, Y., Song, R., Li, C., Li, J., & Gao, J. (2023). The roles of gibberellins in regulating leaf development. Plants, 12(6), 1243. https://doi.org/10.3390/plants12061243
Şahin, N. K., Okumuş, O., & Say, A. (N. D.) Role of gibberellic acid (GA3) in seed germination and early seedling development in some field crops: A review. MAS Journal of Applied Sciences. https://doi.org/10.5281/zenodo.15082178
Shashibhushan, D., Reddy, M. A., Bhadru, D., & Pradeep, T. (2021). Effect of gibberellic acid (GA3) on the yield-attributing traits during a cold period in rice. International Journal of Environment and Climate Change, 11(5), 34-38.
Shu, K., Liu, X., Xie, Q., & He, Z. (2015). Two faces of one seed: Hormonal regulation of dormancy and germination. Molecular Plant, 8(8), 1121-1133. https://doi.org/10.1016/j.molp.2015.08.010
Spies, L. (2023). The effect of gibberellic acid concentration on Gentiana andrewsii germination. University of Northern Iowa. https://scholarworks.uni.edu/hp/
Wang, J. D., Wang, J., Huang, L. C., Kan, L. J., Wang, C. X., Xiong, M., & Li, Q. F. (2024). ABA-mediated regulation of rice grain quality and seed dormancy via the NF-YB1-SLRL2-bHLH144 Module. Nature Communications, 15, Article number: 4493.
Xu, F., Yoshida, H., Chu, C., Matsuoka, M., & Sun, J. (2025). Seed dormancy and germination in rice: Molecular regulatory mechanisms and breeding. Molecular Plant, 18(6), 960-977. https://doi.org/10.1016/j.molp.2025.05.010
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